Prot —&gt; mRNA —&gt; cDNA —&gt; dsDNA. (Step 2 via reverse transcriptase and step 3 via DNA Polymerase). Now the dsDNA is injected into a virus which infects bacteria to make lots of the DNA.

8:3. DNA Libraries and Generating cDNA

Denaturation (Heat to high temp), Primer Annealing (Cool slightly), Primer Extension via polymerase (Heat but not as much as step 1).

8:2. Polymerase Chain Reaction

GE find us the length of a DNA Strand (how many base pairs long).

8:1. Gel Electrophoresis

Not really sure why I did a module on this - basically you know they’re homo recessive because they have blue eyes (the recessive trait), and you know to use q BECAUSE we’re dealing with the recessive trait.

7:5. Applying the HP Equation

HP Equation gives P(allele frequencies). P^2 is probability of homo dominant, Q^2 is probability of homo recessive, 2pq is probability of heterozygous allele combo. P = frequency of dom allele, Q = frequency of recessive allele.

7:4. The Hardy-Weinberg Equation

Discusses Dihybrid Crosses and the Law of Independent Assortment.

7:3. Worked Example: Punnett Squares

Don’t let the title fool you. This is short. Discusses complete, incomplete, and co-dominance.

7:2. Co-dominance and Incomplete Dominance

Discuss genes, alleles, geno/phenotypes, and punnet squares. The Khan Academy video discusses the history (Gregor Mendel) but let’s be real, who cares.

7:1. An Introduction to Mendelian Genetics

6:5. The Effects of Mutations

Define mutagens and then discuss endogenous and exogenous mutagens. (Endo: ROS); (Exo: intercalators, base analogues, carcinogens)

6:4. Mutagens and Carcinogens

A concise review of the Khan Academy MCAT Videos.Ok this is really random but I just hit a thousand plays or this, so could you hmu with any critique? Email mcatstudyone@gmail.com (takes 20 seconds!)Created by Hamza Taj

Small scale mutations (point: TTM; frame-shift: ID) and Large-scale mutations (translocation, inversion).

6:3. The Causes of Genetic Mutations

Point, Frame Shift, and Nonsense vs. Missense. Further divided Missense to Silent, Conservative, and Non-Conservative.

6:2. The Different Types of Mutations

Clarifies that mutations arise at the DNA level, and from where mutations originate.

6:1. Introduction to Genetic Mutations

Discusses categories of tumor suppressors and then 2 examples: pRb and p53, both proteins.

5:7. Tumor Suppressors

Discusses the 3 mechanisms through which oncogenes are formed from proto-oncogenes, and types of oncogenes with examples.

5:6. Oncogenes

I discuss ncRNAs (RNA that’s ready to perform tasks w/o becoming protein) and then move onto rRNA, tRNA, miRNA, snRNA, and snoRNA. Have fun with all these acronyms lol

5:5. Non-Coding RNA

Not really an important module but skip to 6:18 for new stuff (RNA Editing via the ADAR and CDAR Enzymes)

5:4. Post-Transcriptional Regulation

Discusses GTFs, then discusses activators/repressors and enhancers/silencers. Finally distinguishes between regulation of transcriptional euk and prok organisms.

5:3. Regulation of Transcription

Discusses HAT and HDAC enzymes to uncoil/coil DNA (uncoil = activate for transcription) then transitioned into DNA methylation as it relates to CpG Islands and MBB’s.

5:2. DNA and Chromatin Regulation

More terminology of the Lac Operon mechanism, refined ideas from the first part.

5:1. The Lac Operon - Part 2

A concise review of the Khan Academy MCAT Videos.<br><br>Ok this is really random but I just hit a thousand plays or this, so could you hmu with any critique? Email mcatstudyone@gmail.com (takes 20 seconds!)<br><br>Created by Hamza Taj

MCAT Modules - Review

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