A teaching assistant explains the process of transformation and protein expression, using the example of human insulin expressed in bacterial cells.

Transformation and Protein Expression

A teaching assistant explains the polymerase chain reaction (PCR), and examples of its common uses.

Polymerase Chain Reaction (PCR)

A teaching assistant works through a practice problem on pedigree charts.

Pedigrees

A teaching assistant explains how to construct genomic libraries and cDNA libraries.

Genomic and cDNA Libraries

A teaching assistant works through a practice problem on transcription and translation.

Transcription & Translation

A teaching assistant explains the lac operon, using props to demonstrate how the lactose metabolism machinery works.

Lac operon

A teaching assistant works through a practice problem on Complementation.

Complementation (Part II)

A teaching assistant explains the concept of complementation using knitting needles and yarn to demonstrate.

Complementation

A teaching assistant explains the numbering and labeling conventions of nucleic acids and proteins.

Explanation of 5' and 3', C terminus, and N terminus

Professor Lander continues with the discussion of DNA Sequencing technologies, methods of analyzing DNA sequencing data, and the process of Polymerase Chain Reaction (PCR) and its applications.

Agarose Gel Electrophoresis, DNA Sequencing, PCR, Excerpt 2

OCW Scholar: Fundamentals of Biology

Professor Walker gives an overview of glycolysis, respiration, and fermentation, and how cells use the energy from these processes.

Glycolysis, Respiration, and Fermentation

Professor Walker explains the process of photosynthesis, its evolutionary significance, and the structure of chloroplasts.

Photosynthesis

Professor Walker discusses the chemical forces that are important for the structure and function of biomolecules, and how these properties influence the characteristics of key macromolecules.

Covalent Bonds, Hydrogen Bonds

Professor Sive introduces macromolecules, and discusses the structures, properties, and functions of lipids and carbohydrates.

Macromolecules: Lipids, Carbohydrates, Nucleic Acid, Excerpt 1

A teaching assistant goes over a practice problem on lipids, carbohydrates, and nucleic acids.

Lipids, Carbohydrates, and Nucleic Acids Practice Problem

Professor Walker explains the categories of amino acids, the levels of protein structure, the bonds and forces in each level, and how they can influence protein structure.

Proteins, levels of Structure, Non-covalent Forces, excerpt 2

Professor Lander explains methods of analyzing a gene of interest after it has been cloned.

Agarose Gel Electrophoresis, DNA Sequencing, PCR, Lecture Video Excerpt 1

Professor Lander reviews the process of constructing a DNA library, and then discusses how to analyze and use the information in the library.

Constructing and Screening a Recombinant DNA Library

A teaching assistant goes over a practice problem on covalent bonds, chirality, electronegativity, and hydrogen bonds.

Covalent Bonds

Professor Lander discusses variations in the processes of DNA replication, transcription, and translation among eukaryotes, prokaryotes, and viruses.

Alternative Approaches to Molecular Biology

Professor Sive discusses the chemical reactions within a cell by explaining the rules underlying energy use, how enzymes and catalysts work, and how ATP is used in cells.

Biochemical Reactions, Enzymes, and ATP

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