Adam Blattler's guest appearances

Adam Blattler

Epigenetics Podcast

Joe Fernandez

David Jones

Monica Dus

Karmella Haynes

Erez Lieberman Aiden

Noe Gonzalez

Shelley Berger

Emily Bernstein

Wendy Bickmore

Gary Karpen

Susan Gasser

Henk Stunnenberg

Peter Tessarz

Don Olins

Markus Buschbeck

OwlTail

Chromatin Immunoprecipitation (ChIP) was first developed in Drosophila, where interactions of RNA Pol II with genes were investigated (Mol. Cell. Biol. August 1985 vol. 5 no. 8 2009-2018). Then, in a 1993 publication, (Genes &amp; Dev. 1993 7:592-604), the group of James Broach described the association of histone acetylation state with transcriptional gene silencing in yeast. The technique was first used successfully in mammalian cells by Richard Treisman's group, published in 1998 (Cell (1998) 92:475-87).  Chromatin Immunoprecipitation is used to link specific states of chromatin to individual loci in a cell, to understand how genes are regulated, and to decipher the Histone Code. In this Episode, we discuss the multiple challenges of ChIP experiments and the difficulties that can arise during different steps of the process. References for this episode Epigenetics News: Chung-Chau Hon, Jordan A. Ramilowski, … Alistair R. R. Forrest. An atlas of human long non-coding RNAs with accurate 5′ ends. Nature. March 2017. doi:10.1038/nature21374 Jennifer L. Guerriero, Alaba Sotayo, … Anthony Letai. Class IIa HDAC inhibition reduces breast tumours and metastases through anti-tumour macrophages. Nature. March 2017. doi:10.1038/nature21409 Nitika Taneja, Martin Zofall, … Shiv I. S. Grewal. SNF2 Family Protein Fft3 Suppresses Nucleosome Turnover to Promote Epigenetic Inheritance and Proper Replication. Molecular Cell. April 2017. DOI:10.1016/j.molcel.2017.02.006  Multiple Challenges in ChIP D. S. Gilmour, J. T. Lis. In vivo interactions of RNA polymerase II with genes of Drosophila melanogaster. Molecular and Cellular Biology. August 1985. doi:10.1128/MCB.5.8.2009 M. Braunstein, A. B. Rose, … J. R. Broach. Transcriptional silencing in yeast is associated with reduced nucleosome acetylation. Genes &amp; Development. 1993. doi:10.1101/gad.7.4.592 Arthur S. Alberts, Olivier Geneste, Richard Treisman. Activation of SRF-Regulated Chromosomal Templates by Rho-Family GTPases Requires a Signal that Also Induces H4 Hyperacetylation. Cell. February 1986. doi:10.1016/S0092-8674(00)80941-1  Active Motif Contact Details Follow us on Twitter Join us on LinkedIn Like us on Facebook Email us @Active Motif Europe or Active Motif North America. 

Chromatin Immunoprecipitation (ChIP) was first developed in Drosophila, where interactions of RNA Pol II with genes were investigated (Mol. Cell. Biol. August 1985 vol. 5 no. 8 2009-2018). Then, in a 1993 publication, (Genes &amp; Dev. 1993 7:592-604), the group of James Broach described the association of histone acetylation state with transcriptional gene silencing in yeast. The technique was first used successfully in mammalian cells by Richard Treisman's group, published in 1998 (Cell (1998) 92:475-87). Chromatin Immunoprecipitation is used to link specific states of chromatin to individual loci in a cell, to understand how genes are regulated, and to decipher the Histone Code. In this Episode, we discuss the multiple challenges of ChIP experiments and the difficulties that can arise during different steps of the process. References for this episode Epigenetics News: <ul><li>Chung-Chau Hon, Jordan A. Ramilowski, … Alistair R. R. Forrest. <a href="http://www.nature.com/nature/journal/v543/n7644/full/nature21374.html" rel="nofollow" target="_blank">An atlas of human long non-coding RNAs with accurate 5′ ends</a>. Nature. March 2017. doi:10.1038/nature21374</li> <li>Jennifer L. Guerriero, Alaba Sotayo, … Anthony Letai. <a href="http://www.nature.com/nature/journal/v543/n7645/full/nature21409.html" rel="nofollow" target="_blank">Class IIa HDAC inhibition reduces breast tumours and metastases through anti-tumour macrophages</a>. Nature. March 2017. doi:10.1038/nature21409</li> <li>Nitika Taneja, Martin Zofall, … Shiv I. S. Grewal. <a href="http://www.cell.com/molecular-cell/abstract/S1097-2765(17)30098-9" rel="nofollow" target="_blank">SNF2 Family Protein Fft3 Suppresses Nucleosome Turnover to Promote Epigenetic Inheritance and Proper Replication</a>. Molecular Cell. April 2017. DOI:10.1016/j.molcel.2017.02.006</li> </ul> Multiple Challenges in ChIP <ul><li>D. S. Gilmour, J. T. Lis. <a href="http://mcb.asm.org/content/5/8/2009" rel="nofollow" target="_blank">In vivo interactions of RNA polymerase II with genes of Drosophila melanogaster</a>. Molecular and Cellular Biology. August 1985. doi:10.1128/MCB.5.8.2009</li> <li>M. Braunstein, A. B. Rose, … J. R. Broach. <a href="http://genesdev.cshlp.org/content/7/4/592" rel="nofollow" target="_blank">Transcriptional silencing in yeast is associated with reduced nucleosome acetylation.</a> Genes &amp; Development. 1993. doi:10.1101/gad.7.4.592</li> <li>Arthur S. Alberts, Olivier Geneste, Richard Treisman. <a href="http://www.cell.com/cell/abstract/S0092-8674(00)80941-1" rel="nofollow" target="_blank">Activation of SRF-Regulated Chromosomal Templates by Rho-Family GTPases Requires a Signal that Also Induces H4 Hyperacetylation</a>. Cell. February 1986. doi:10.1016/S0092-8674(00)80941-1</li> </ul> Active Motif Contact Details <ul><li>Follow us on <a href="https://twitter.com/activemotif" rel="nofollow" target="_blank">Twitter</a></li> <li>Join us on <a href="https://www.linkedin.com/company-beta/35651/" rel="nofollow" target="_blank">LinkedIn</a></li> <li>Like us on <a href="https://www.facebook.com/ActiveMotifInc/" rel="nofollow" target="_blank">Facebook</a></li> <li>Email us @<a href="mailto:eurotech@activemotif.com?Subject=Podcast%20inquiry">Active Motif Europe</a> or <a href="mailto:sales@activemotif.com?Subject=Podcast%20inquiry">Active Motif North America</a>.</li> </ul>

Multiple Challenges in ChIP (Adam Blattler)